Inhibitors

-isobutyl-1-methylxanthine: inhibits: phosphodiesterase [ pdgf01464.html ]

Moreover , a phosphodiesterase inhibitor , -isobutyl-1-methylxanthine , and a more specific cAMP phosphodiesterase inhibitor , Ro 20-1724 , augmented the inhibition of DNA synthesis in SMC concomitant with further elevation of cAMP level .
2',5'-dideoxyadenosine: inhibits: adenylyl cyclase [ pdgf01597.html ]

5 . The antiproliferative action of A02011-1 was mimicked by 8-bromo-cyclic AMP , a membrane-permeable cyclic AMP analogue and was antagonized by 2',5'-dideoxyadenosine , an adenylyl cyclase inhibitor and by Rp-cyclic AMPS , a competitive inhibitor of cyclic AMP-dependent protein kinase ( PKA ) type I and II .
2-aminopurine: inhibits: chemical [ pdgf00509.html ]

Previous reports have shown that 1 ) dsRNA , a direct activator of PKR , induces the genes c-myc , c-fos , and JE ; 2 ) 2-aminopurine , a chemical inhibitor of PKR , blocks the induction of these genes by serum ; and 3 ) activated p21ras induces a cellular inhibitor of PKR .
25-hydroxycholesterol: inhibits: HMG CoA reductase [ pdgf01932.html ]

It was found that short transient exposures to the HMG CoA reductase inhibitor 25-hydroxycholesterol temporarily blocked the cell cycle traverse in the postmitotic half of G1 ( G1pm ) , whereas cells in the subsequent cell cycle phases were unaffected .
5,6-dichloro-1 beta-d-ribofuranosylbenzimidazole: inhibits: RNA II polymerase transcription [ pdgf00189.html ]

The data indicate that the t1/2 of PDGF-alpha receptor mRNA is approximately 3.3 h after incubation with the RNA II polymerase transcription inhibitor 5,6-dichloro-1 beta-D-ribofuranosylbenzimidazole ( DRB ) .
6-dichloro-1-beta-d-ribofuranosyl benzimidazole: inhibits: RNA polymerase II [ pdgf01639.html ]

In order to decide if the u-PAR mRNA increase was due to message stabilization or a consequence of transcriptional activation we used the RNA polymerase II inhibitor , 6-dichloro-1-beta-D-ribofuranosyl benzimidazole ( DRB ) during the stimulation experiments .
6-dichlorobenzimidazole riboside: inhibits: RNA polymerase [ pdgf00596.html ]

Full repression of p27kip1 synthesis required the continued presence of PDGF and failed to occur in the presence of the RNA polymerase inhibitor , 6-dichlorobenzimidazole riboside .
CGP: inhibits: specific PKC [ pdgf00980.html ]

The late phase of the time-dependent epidermal growth factor ( EGF)-induced biphasic activation of the p70s6k is selectively attenuated by the specific PKC inhibitor , CGP 41,251 , a staurosporine derivative .
GF: inhibits: protein kinase C [ pdgf00311.html ]

This decrease in proliferation as well as the change in morphology of the TPA-treated cells can be blocked by the protein kinase C inhibitor GF 109203X .
H7: inhibits: serine/threonine protein kinase [ pdgf00086.html ]

The serine/threonine protein kinase inhibitor , H7 , blocked PDGF A and B chain mRNA induction in response to TGF-beta .; inhibits: PKC [ pdgf00338.html ]

In contrast to the effect of PMA , the inactive analog phorbol had no effect on PDGF-A chain mRNA levels , while the PKC inhibitor H7 markedly reduced the PMA-induced increment in PDGF-A chain mRNA .
IBMX: inhibits: phosphodiesterase [ pdgf00304.html ]

Incubating cells with PDGF and -isobutyl-1-methylxanthine ( IBMX , a phosphodiesterase inhibitor ) enhanced the accumulation of cAMP and PKA activity by an additional . 5-3-fold , whereas IBMX alone was essentially without effect .
U-71322: inhibits: phospholipase C [ pdgf00492.html ]

The chemotactic response of F5 met was inhibited by both the PI 3-kinase inhibitor wortmannin and the phospholipase C inhibitor U-71322 .
W-7: inhibits: specific CaM [ pdgf01519.html ]

Trifluoperazine and W-7 , both specific CaM inhibitors S , similarly inhibited DNA synthesis stimulated by EGF , PDGF or FBS in a dose-dependent manner , whereas W-5 , a less specific CaM inhibitor , was minimally effective .
actinomycin D: inhibits: transcription [ pdgf01816.html ]

The transcription inhibitor , actinomycin D , and protein synthesis inhibitor , cycloheximide , inhibited the increase in GM-CSF and G-CSF production induced by IL-1 and TNF .
alpha-amanitin: inhibits: RNA polymerase II [ pdgf00315.html ]

alpha-Amanitin , an RNA polymerase II inhibitor , blocked transcription by more than 70% .; inhibits: RNA synthesis [ pdgf01865.html ]

The induction of TH by muscle extract was ( 1 ) dose dependent ; ( 2 ) paralleled by a proportional increase in the steady-state levels of TH mRNA ; ( 3 ) greatly reduced by the RNA synthesis inhibitor alpha-amanitin or the protein synthesis inhibitor cycloheximide ; and ( 4 ) unassociated with change in the survival of neurons in culture .
calphostin c.: inhibits: protein kinase C [ pdgf00298.html ]

However , stimulation of c-fos was slightly more sensitive to genistein , while the B2 receptor mRNA was more sensitive to inhibition by the protein kinase C inhibitor , calphostin C. The increase in cell surface B2 receptors were functionally coupled to an increase in phosphoinositide-specific phospholipase C , and the effects of PDGF were selective as there was no increase in either angiotensin II- or arginine vasopressin-induced inositol phosphate formation or intracellular calcium release .
cilostazol: inhibits: cyclic AMP phosphodiesterase [ pdgf01725.html ]

Cilostazol , a cyclic AMP phosphodiesterase inhibitor , has been used as an antiplatelet agent .; inhibits: cyclic AMP phosphodiesterase [ pdgf01725.html ]

Effect of cilostazol , a cyclic AMP phosphodiesterase inhibitor , on the proliferation of rat aortic smooth muscle cells in culture .
cycloheximide: inhibits: protein synthesis [ pdgf00002.html ]

The effects of PDGF on PDGF-A mRNA and polypeptide levels were prevented by the protein synthesis inhibitor cycloheximide at 3.6 microM .; inhibits: protein-synthesis [ pdgf00097.html ]

The presence of a protein-synthesis inhibitor , cycloheximide , in the culture medium did not significantly influence the PDGF A-chain mRNA level in normal mesothelial and malignant mesothelioma cell lines .; inhibits: protein synthesis [ pdgf00165.html ]

PDGF A-chain mRNA levels increase when quiescent HUVE cells are treated with the protein synthesis inhibitor cycloheximide ; therefore , the effect of cycloheximide on PDGF A-chain mRNA decay was also investigated .; inhibits: protein synthesis [ pdgf00218.html ]

HBGF-1 induction of PDGF A-chain mRNA expression occurs in the presence of the protein synthesis inhibitor cycloheximide and thus does not require de novo protein synthesis .; inhibits: protein synthesis [ pdgf00772.html ]

A rapid increase in activity ( maximal at min ) is followed by a lower persistent level of activity which is maximal at 4-6 h . The second peak of MAP kinase activity is markedly attenuated by the protein synthesis inhibitor cycloheximide and , consequently , is paralleled by a marked de-novo synthesis of p42 and p44 MAP kinases , as measured by immunoprecipitation of [ 35S]methionine-labeled mesangial cells and by a 700% increase in total MAP kinase protein , as detected by Western-blot analysis .; inhibits: protein synthesis [ pdgf01295.html ]

The protein synthesis inhibitor , cycloheximide ( 10 micrograms/ml ) , also increases the level of urokinase mRNA .
dazmegrel: inhibits: thromboxane A2 synthetase [ pdgf00739.html ]

In order to examine ( indirectly ) the role of PG in mediating changes in MC DNA synthesis , indomethacin ( 1.5 x 10(-5 ) mol/l ) or the thromboxane A2 synthetase inhibitor Dazmegrel ( 10(-5 ) mol/l ) was added to the medium and DNA synthesis assessed after 72 h using 3H-thymidine incorporation ( 3H-TdR ) .
drug suramin: inhibits: growth factor [ pdgf01137.html ]

In contrast , we found that AII-stimulated mitogenesis could be inhibited in a dose-dependent manner by the growth factor inhibitor drug suramin .
enalapril: inhibits: angiotensin-converting enzyme [ pdgf01130.html ]

We have now assessed the effect of the angiotensin-converting enzyme inhibitor enalapril on the expression of the ET-1 , TNF-alpha , PDGF-B , TGF-beta , and bFGF genes in 24-week-old rat glomeruli after streptozotocin injection .
erbstatin: inhibits: natural tyrosine kinase [ pdgf01190.html ]

The natural tyrosine kinase inhibitor erbstatin and a synthetic analog ( 1302 ) were co0pared for their inhibitory activity on EGF receptor kinase , PDGF receptor kinase and a src-type kinase from bovine brain .
finasteride: inhibits: 5-alpha reductase [ pdgf01401.html ]

However , when conversion of T to DHT was blocked by the 5-alpha reductase inhibitor finasteride , the levels of AR mRNA were considerably down-regulated by T ( 10-500 nM ) , particularly in the contact-inhibited cells .
genistein: inhibits: tyrosine kinase [ pdgf00160.html ]

Genistein , a tyrosine kinase inhibitor , inhibited in a dose-related manner ( 2-20 microM ) the mitogenic effect induced by either PDGF or the combination of vanadate and PDGF .; inhibits: tyrosine kinase [ pdgf01110.html ]

Genistein , a tyrosine kinase inhibitor , inhibited PLD activation but not DGK activation , the latter was inhibited selectively by R 59022 .; inhibits: tyrosine kinase [ pdgf00542.html ]

In the present study , we investigated the effects of genistein , a tyrosine kinase inhibitor , and NiCl2 , a Ca2+ influx blocker , on PDGF-induced Ca2+ transient and on expression of c-fos and c-myc mRNA .; inhibits: specific tyrosine protein kinase [ pdgf00566.html ]

The inductions were abolished when genistein , a specific tyrosine protein kinase inhibitor , was added concomitantly with PDGF , a condition in which DNA synthesis is known to be inhibited .; inhibits: TPK [ pdgf00570.html ]

The TPK inhibitor genistein blocked PDGF induction of these events , including DNA synthesis , with the exception of receptor down regulation .; inhibits: tyrosine kinase [ pdgf00832.html ]

The tyrosine kinase inhibitor genistein dose-dependently antagonized the inhibitory effect of PDGF-BB on IL-1 beta-stimulated PLA2 secretion , thus suggesting that tyrosine phosphorylation may be required for PDGF-BB inhibition of PLA2 gene expression in mesangial cells .; inhibits: tyrosine kinase [ pdgf00948.html ]

Another tyrosine kinase inhibitor genistein also inhibited reorganization of F-actin .; inhibits: tyrosine kinase [ pdgf01491.html ]

We have examined the effects of the tyrosine kinase inhibitor genistein on hormone dependent cell proliferation and intracellular signalling in mouse 10T1/2 fibroblasts and rat liver T51B epithelial cells .
herbimycin: inhibits: tyrosine kinase [ pdgf01371.html ]

PDGF-induced beta-actin mRNA redistribution was inhibited by the tyrosine kinase inhibitor herbimycin , indicating that this process requires intact tyrosine kinase activity , similar to actin filament polymerization and chemotaxis .
herbimycin-a: inhibits: tyrosine kinase [ pdgf00948.html ]

In this communication , we demonstrate that the tyrosine kinase inhibitor herbimycin-A ( HM-A ) inhibited the effects of PDGF on SMCs in proliferation , phenotypic modulation , and reorganization of F-actin .
indomethacin: inhibits: cyclooxygenase [ pdgf00304.html ]

The PDGF-stimulated increase in cAMP was prevented by addition of the cyclooxygenase inhibitor indomethacin , consistent with release of prostaglandins stimulating cAMP .; inhibits: prostaglandin synthesis [ pdgf00359.html ]

IL-1 beta stimulates PDGF-AA synthesis also in the presence of indomethacin , a prostaglandin synthesis inhibitor .; inhibits: prostaglandin H synthase [ pdgf00854.html ]

The addition of indomethacin , a prostaglandin H synthase inhibitor , or nordihydroguaiaretic acid , a lipoxygenase inhibitor , did not affect PDGF-stimulated thymidine uptake into DNA .
inter-alpha-inhibitor: inhibits: serine protease [ pdgf01563.html ]

OBJECTIVE : The hyaluronan-binding protein TSG-6 ( tumor necrosis factor-stimulated gene 6 ) forms a stable complex with the serine protease inhibitor , inter-alpha-inhibitor , potentiates the inhibition of plasmin activity , and has antiinflammatory effects in vivo .
k252a: inhibits: efficient serine/threonine protein kinase [ pdgf01764.html ]

K252a , an efficient serine/threonine protein kinase inhibitor ( IC50s of to 30 nM ) , has been shown to block the neuronal differentiation of rat pheochromocytoma PC12 cells induced by nerve growth factor ( NGF ) .
lisinopril: inhibits: angiotensin-converting enzyme [ pdgf00325.html ]

In this study , we tested whether lisinopril , an angiotensin-converting enzyme inhibitor , or verapamil , a calcium channel blocker , would suppress the PDGF gene expression in stimulated human saphenous vein ECs .
lovastatin: inhibits: HMG-CoA reductase [ pdgf01575.html ]

Recipients treated with the HMG-CoA reductase inhibitor lovastatin , which inhibits isoprenoid production , showed significantly decreased allograft intimal area after weeks , when compared with untreated recipients .
nih3t3 cells: inhibits: serine/ threonine phosphatase [ pdgf01545.html ]

In NIH3T3 cells , the serine/ threonine phosphatase inhibitor okadaic acid and the tyrosine kinase inhibitor genistein increased Shb mRNA contents , the protein kinase C activating phorbol ester 12-O-tetradecanoyl -acetate ( TPA ) decreased the Shb mRNA content , whereas the tyrosine kinase inhibitor tyrphostin 25 and the mitogen platelet-derived growth factor ( PDGF-BB ) had no effect .
nitrobenzylthioinosine: inhibits: nucleoside transport [ pdgf00548.html ]

Experiments with the nucleoside transport inhibitor nitrobenzylthioinosine suggested that the transport of c3Ado into EC was required for its inhibitory activity .
ocadaic acid: inhibits: potent phosphatase [ pdgf01127.html ]

This effect could be augmented by the addition of ocadaic acid , a potent phosphatase inhibitor , suggesting that protein phosphorylation plays a role in this process .
okadaic acid: inhibits: phosphatase [ pdgf01648.html ]

We now report evidence that the serine/threonine kinase inhibitors H7 and H8 but not HA1004 , W-7 , and ML-7 inhibit the transcriptional induction of the JE gene by serum whereas the phosphatase inhibitor , okadaic acid , increases JE expression .
pervanadate: inhibits: tyrosine phosphatase [ pdgf00924.html ]

Treatment with the tyrosine phosphatase inhibitor pervanadate further increased PLC gamma 2 tyrosine phosphorylation as well as [ 3H]IP2 and [ 3H]IP3 generation .
ro 31-8220: inhibits: selective protein kinase C [ pdgf01302.html ]

A selective protein kinase C inhibitor , Ro 31-8220 , abolished induction of the 95 kDa gelatinase .
ro-31-8220: inhibits: protein kinase C [ pdgf00185.html ]

Treatment of the cells with the protein kinase C inhibitor , Ro-31-8220 , and depletion of cellular protein kinase C by pretreatment with 4 beta-phorbol 12-myristate 13-acetate resulted in loss of PLD activation by PDGF indicating a PKC-dependent mechanism .; inhibits: PKC [ pdgf01065.html ]

Moreover , exposure to Ro-31-8220 , a PKC inhibitor , had significantly greater effects on the response to PMA than on that to bombesin .
sodium orthovanadate: inhibits: phosphatase [ pdgf00226.html ]

The inhibitory effect of suramin on receptor conformation was abolished by the phosphatase inhibitor , sodium orthovanadate , suggesting that receptor phosphorylation mediated the conformational change .; inhibits: phosphatase [ pdgf00277.html ]

The inhibitory effect of suramin on receptor conformation was abolished by the phosphatase inhibitor sodium orthovanadate , suggesting that receptor phosphorylation mediated the conformational change .; inhibits: phosphatases [ pdgf01765.html ]

Phosphorylation of p175 is not induced by treatment of the cells with the phosphatases inhibitor sodium orthovanadate .
something: inhibits: ubiquitous proteinase [ pdgf00051.html ]

Alpha 2-Macroglobulin ( alpha 2M ) is a multifunctional secreted glycoprotein that serves as a ubiquitous proteinase inhibitor and as a binding protein for platelet-derived growth factor ( PDGF ) BB and homologues of PDGF-BB secreted in culture by macrophages .; inhibits: proteinase [ pdgf00111.html ]

In this study , we demonstrate that the proteinase inhibitor and cytokine carrier alpha 2-macroglobulin ( alpha 2M ) increases rat VSMC PDGF alpha-receptor expression .; inhibits: PDGF receptor-associated tyrosine kinase [ pdgf00142.html ]

Furthermore , the strain-induced stimulatory effect on DNA synthesis of fetal lung cells was blocked by tyrphostin 9 ( 1 microM ) , a PDGF receptor-associated tyrosine kinase inhibitor , but not by its inactive structural analogue tyrphostin 1 . Antisense but not sense PDGF-beta-R ON ( 10 microM ) also abrogated the strain-enhanced DNA synthesis .; inhibits: proteinase [ pdgf00197.html ]

Therefore , under normal physiological conditions , native alpha 2M ( non-alpha 2M-R/LRP-recognized ) is the primary form of the proteinase inhibitor functioning as a carrier of PDGF-BB and TGF-beta 1 in the blood .; inhibits: angiotensin-converting enzyme [ pdgf00325.html ]

Both angiotensin-converting enzyme inhibitors S and calcium channel blockers decrease postinjury intimal thickening in vivo , but their mechanisms of inhibitory action are unclear .; inhibits: nonspecific lipoxygenase [ pdgf00401.html ]

Pretreatment with either the nonspecific lipoxygenase inhibitor ( nordihydroguaiaretic acid ) or specific inhibitors of 5-lipoxygenase ( SC-41661 and ICI-230487 ) inhibited PDGF-induced mitogenesis .; inhibits: lipoxygenase [ pdgf00401.html ]

The PDGF-induced signal transduction cascade was studied to determine the potential mechanism of action of the lipoxygenase inhibitors S .; inhibits: potent growth [ pdgf00436.html ]

Transforming growth factor-beta 1 ( TGF-beta 1 ) is a potent growth inhibitor for many cell types .; inhibits: protein kinase C [ pdgf00439.html ]

The protein kinase C inhibitors S , H7 and staurosporine , strongly inhibited the shear-induced PDGF gene expression in HUVEC .; inhibits: cyclooxygenase [ pdgf00439.html ]

The cyclooxygenase inhibitors S , ibuprofen and indomethacin , were slightly inhibitory .; inhibits: protein kinase [ pdgf00447.html ]

The protein kinase inhibitors S , staurosporine and K252a , block PDGF-induced ISRE binding and -5A synthetase gene expression .; inhibits: phosphodiesterase [ pdgf00457.html ]

Treatment with forskolin ( an activator of adenylate cyclase ) , IBMX ( a phosphodiesterase inhibitor ) , PGE1 , and isoproterenol stimulated accumulation of both cAMP and c-myc RNA , but no increase in either cAMP or c-myc RNA was seen with the inactive forskolin analog , 9-dideoxyforskolin .; inhibits: Cdk [ pdgf00491.html ]

Differential modulation of G1 cyclins and the Cdk inhibitor p27kip1 by platelet-derived growth factor and plasma factors in density-arrested fibroblasts .; inhibits: Cdk [ pdgf00491.html ]

Density-arrested fibroblasts contain low levels of cyclins E and A , and high levels of the Cdk inhibitor p27kip1 .; inhibits: protein-tyrosine kinase [ pdgf00507.html ]

Selective inhibition of the platelet-derived growth factor signal transduction pathway by a protein-tyrosine kinase inhibitor of the -phenylaminopyrimidine class .; inhibits: potent protein-tyrosine kinase [ pdgf00507.html ]

We describe a potent protein-tyrosine kinase inhibitor ( CGP 53716 ) that shows selectivity for the PDGF receptor in vitro and in the cell .; inhibits: tyrosine protein kinase [ pdgf00566.html ]

Inhibition of PDGF-induced c-jun and c-fos expression by a tyrosine protein kinase inhibitor .; inhibits: growth [ pdgf00568.html ]

Transforming growth factor-beta ( TGF-beta ) acts as a growth inhibitor , yet it can stimulate proliferation ; 1-2 fg/cell of TGF-beta 1 elicits maximal proliferation of dense and sparse cultured smooth muscle cells ( SMCs ) , whereas higher amounts are less stimulatory .; inhibits: cyclin-dependent kinase [ pdgf00596.html ]

We have investigated the regulation of p27kip1 , a cyclin-dependent kinase inhibitor , in BALB/c 3T3 cells during growth factor-stimulated transition from quiescence ( G0 ) to a proliferative ( G1 ) state .; inhibits: protein kinase C [ pdgf00617.html ]

The effects of the protein kinase C inhibitors S on proliferation could not be ascribed to cytotoxicity .; inhibits: physiologic growth [ pdgf00680.html ]

None of these stimulated melanoma cell growth consistently , whereas exogenous , acid-activated TGF- beta inhibited melanoma growth at concentrations greater than 10 ng/ml , suggesting that bioactive TGF- beta may represent a physiologic growth inhibitor .; inhibits: reductase [ pdgf00762.html ]

A reductase inhibitor , we evaluated the effects of lovastatin on DNA replication and the proliferation of rat mesangial and aortic smooth muscle cells , both of which were mesenchymal origin cells .; inhibits: powerful growth [ pdgf00879.html ]

Heparin is a powerful growth inhibitor for SMC .; inhibits: tyrosine kinase [ pdgf00948.html ]

Since both tyrosine kinase inhibitors S reduced PDGF-dependent tyrosine phosphorylation , tyrosine kinase activities may be involved not only in proliferation but also in reorganization of F-actin .; inhibits: synthetic matrix metalloproteinase [ pdgf01072.html ]

Regulation of vascular smooth muscle cell migration and proliferation in vitro and in injured rat arteries by a synthetic matrix metalloproteinase inhibitor .; inhibits: synthetic MMP [ pdgf01072.html ]

To assess the role of MMPs in SMC proliferation and migration and intimal thickening , we measured the effect of the synthetic MMP inhibitor BB94 ( Batimastat ) on DNA synthesis and migration of SMCs in vitro as well as the formation of a neointima after balloon injury to the rat carotid artery .; inhibits: synthase [ pdgf01162.html ]

The effects of hypoxia and the NO synthase inhibitor on ET-1 expression are additive .; inhibits: weaker ACE [ pdgf01185.html ]

Although tuna AI was a weaker ACE inhibitor than captopril , the increasing effect of tuna AI on the migration and the IL-1 generation in BAECs was slightly greater than that of captopril .; inhibits: protein synthesis [ pdgf01208.html ]

The protein synthesis inhibitors S cycloheximide and puromycin reversed the glucocorticoid-mediated inhibition and suggested that new protein synthesis was necessary .; inhibits: aromatase [ pdgf01401.html ]

These effects in both types of cultures were inhibited by platelet derived growth factor ( PDGF ) ( 20 ng/ml ) , a growth factor that up-regulates AR mRNA levels , and by fadrozole ( 100 nM ) , an aromatase inhibitor of the T/estrogen conversion .; inhibits: ACE [ pdgf01460.html ]

The ACE inhibitor did not have detectable independent effects on the A-II induced proliferation or gene expression .; inhibits: DNA synthesis [ pdgf01471.html ]

To investigate whether expression of c-sis RNA is regulated in a cell cycle dependent manner , human A172 glioblastoma cells were synchronized by either centrifugal elutriation or chemical blockage with the DNA synthesis inhibitors S hydroxyurea or aphidicolin .; inhibits: protein kinase C [ pdgf01479.html ]

To evaluate the role of protein kinase C-mediated pathways in the proliferation of malignant gliomas , this study examined the effect of a protein kinase C ( PKC)-activating phorbol ester ( 12-O-tetradecanoyl-13-phorbol acetate or TPA ) and a protein kinase C inhibitor ( polymyxin B ) on deoxyribonucleic acid ( DNA ) synthesis of malignant glioma cells in vitro .; inhibits: cyclic nucleotide phosphodiesterase [ pdgf01497.html ]

The mitogenic effects of PMA were potentiated by cycloheximide pretreatment , and they were abolished by 3-isobutyl-1-methyl xanthine ( IBMX ; a cyclic nucleotide phosphodiesterase inhibitor ) .; inhibits: lipoxygenase pathway [ pdgf01663.html ]

We have measured the effect of the lipoxygenase pathway inhibitors S nordihydroguiaretic acid ( NDGA ) , 5,6-dehydroarachidonic acid , and baicalein on bovine capillary endothelial cell ( EC ) and aortic smooth muscle cell ( SMC ) growth in the presence or the absence of growth factors .; inhibits: potent phosphotyrosyl phosphatase [ pdgf01695.html ]

In the present study , we focus our investigation on the similarity between a redox cycling naphthoquinone and orthovanadate ( VO ) , an oxidant generator as well as a potent phosphotyrosyl phosphatase inhibitor , in growth of 3T3-L1 cells cultured in serum-free media .; inhibits: specific cytoplasmic protein tyrosine kinase [ pdgf01702.html ]

Herbimycin A , a specific cytoplasmic protein tyrosine kinase inhibitor , selectively inhibited the growth of src or abl transformed cells in the serum-free medium resulting in about 10-fold or fivefold lower IC50 than those in the serum-containing medium .; inhibits: growth [ pdgf01715.html ]

Our results show that stimulation by serum of dense cultures of 3T3 cells rapidly induced increased synthesis of a growth inhibitor ( mIGFBP-3 ) capable of binding IGF .; inhibits: tissue [ pdgf01743.html ]

Most interestingly , the effects of EGF and TGF-alpha can be completely reversed by the tissue inhibitor of metalloproteinases , TIMP .; inhibits: specific tyrosine kinase [ pdgf01762.html ]

Herbimycin A , a specific tyrosine kinase inhibitor , both decreases thrombin-induced mitogenesis by greater than 90% and abolishes tyrosine phosphorylation of phospholipase C ( PLC)-gamma-1 .; inhibits: PKC [ pdgf01789.html ]

The PKC inhibitors S , staurosporine , sphingosine , and -(-5-iso-quinoline-sulfonyl)-2-methylpiperazine ( H-7 ) also antagonized both proliferation and PKC activation .; inhibits: ACE [ pdgf01810.html ]

Another ACE inhibitor of a different chemical class , captopril , reduced neointima formation as strongly as cilazapril ( 67 and 78% , respectively ) , but the calcium antagonist verapamil was not active as an inhibitor of neointima formation , despite similar lowering of blood pressure .; inhibits: proteinase [ pdgf01921.html ]

In addition to its effects on the secretion of PA , TGF beta enhanced the production of a proteinase inhibitor by these cells .; inhibits: growth [ pdgf01923.html ]

With TGF beta , which is a growth inhibitor for certain epithelial and other cell types , the loss of the normal inhibitory response in transformed cells could have the same result as the activation of a growth-stimulatory response .; inhibits: growth [ pdgf01924.html ]

Thus TGF beta is primarily a growth inhibitor and not a classical growth factor .
staurosporin: inhibits: protein kinase C [ pdgf00303.html ]

The protein kinase C inhibitor , staurosporin , completely suppressed an increase in PDGF-BB binding by high glucose , and high glucose significantly activated protein kinase C. These results indicated that PDGF-beta receptor expression was enhanced by high glucose through the activation of protein kinase C. Furthermore , we observed similar effects of high glucose on both PDGF-beta receptor expression and protein kinase C activation in rat mesangial cells and human capillary endothelial cells .
staurosporine: inhibits: PKC [ pdgf00310.html ]

Prolonged exposure to phorbol myristate acetate ( PMA ) , which depletes protein kinase C ( PKC ) , or staurosporine , a PKC inhibitor , did not block lyso-PC-induced increases in PDGF-B or ICAM-1 mRNA .; inhibits: PKC [ pdgf01178.html ]

The PKC inhibitor , staurosporine , antagonized mitogenesis stimulated by ATP .; inhibits: kinase [ pdgf01512.html ]

The effects of the kinase inhibitor staurosporine on mitogenesis in NIH/3T3 fibroblasts were characterized .
thapsigargin: inhibits: endomembrane Ca2+ ATPase [ pdgf01140.html ]

However , the release of [ Ca2+]i from the intracellular store of the nef-expressing cells by an endomembrane Ca2+ ATPase inhibitor , thapsigargin , revealed that these cells contained normal Ca2+ stores .
tranexamic acid: inhibits: plasmin [ pdgf00758.html ]

Tranexamic acid , a plasmin inhibitor , abrogated the stimulation of SMC migration by IL-4 .
tyrphostin: inhibits: tyrosine kinase [ pdgf00298.html ]

Comparison with the immediate early response gene c-fos demonstrated that the increase in B2 receptor mRNA was similarly inhibited by the tyrosine kinase inhibitor , tyrphostin , as well as staurosporine .; inhibits: tyrosine kinase [ pdgf01084.html ]

Growth factor-mediated induction of ScR activity in rabbit and human SMC was blocked by the tyrosine kinase inhibitor tyrphostin 47 , whereas the induction of ScR activity in rabbit but not human SMC was blocked by the protein kinase C inhibitor MDL.29,152 .
vanadate: inhibits: potent phosphatase [ pdgf00160.html ]

Vanadate , a potent phosphatase inhibitor , induced a twofold increase in thymidine incorporation .

Inhibiteds

5-alpha reductase: inhibited by: finasteride [ pdgf01401.html ]

However , when conversion of T to DHT was blocked by the 5-alpha reductase inhibitor finasteride , the levels of AR mRNA were considerably down-regulated by T ( 10-500 nM ) , particularly in the contact-inhibited cells .
ACE: inhibited by: something [ pdgf01460.html ]

The ACE inhibitor did not have detectable independent effects on the A-II induced proliferation or gene expression .; inhibited by: something [ pdgf01810.html ]

Another ACE inhibitor of a different chemical class , captopril , reduced neointima formation as strongly as cilazapril ( 67 and 78% , respectively ) , but the calcium antagonist verapamil was not active as an inhibitor of neointima formation , despite similar lowering of blood pressure .
PKC: inhibited by: staurosporine [ pdgf00310.html ]

Prolonged exposure to phorbol myristate acetate ( PMA ) , which depletes protein kinase C ( PKC ) , or staurosporine , a PKC inhibitor , did not block lyso-PC-induced increases in PDGF-B or ICAM-1 mRNA .; inhibited by: H7 [ pdgf00338.html ]

In contrast to the effect of PMA , the inactive analog phorbol had no effect on PDGF-A chain mRNA levels , while the PKC inhibitor H7 markedly reduced the PMA-induced increment in PDGF-A chain mRNA .; inhibited by: Ro-31-8220 [ pdgf01065.html ]

Moreover , exposure to Ro-31-8220 , a PKC inhibitor , had significantly greater effects on the response to PMA than on that to bombesin .; inhibited by: staurosporine [ pdgf01178.html ]

The PKC inhibitor , staurosporine , antagonized mitogenesis stimulated by ATP .; inhibited by: something [ pdgf01789.html ]

The PKC inhibitors S , staurosporine , sphingosine , and -(-5-iso-quinoline-sulfonyl)-2-methylpiperazine ( H-7 ) also antagonized both proliferation and PKC activation .
TPK: inhibited by: genistein [ pdgf00570.html ]

The TPK inhibitor genistein blocked PDGF induction of these events , including DNA synthesis , with the exception of receptor down regulation .
adenylyl cyclase: inhibited by: 2',5'-dideoxyadenosine [ pdgf01597.html ]

5 . The antiproliferative action of A02011-1 was mimicked by 8-bromo-cyclic AMP , a membrane-permeable cyclic AMP analogue and was antagonized by 2',5'-dideoxyadenosine , an adenylyl cyclase inhibitor and by Rp-cyclic AMPS , a competitive inhibitor of cyclic AMP-dependent protein kinase ( PKA ) type I and II .
angiotensin-converting enzyme: inhibited by: something [ pdgf00325.html ]

Both angiotensin-converting enzyme inhibitors S and calcium channel blockers decrease postinjury intimal thickening in vivo , but their mechanisms of inhibitory action are unclear .; inhibited by: lisinopril [ pdgf00325.html ]

In this study , we tested whether lisinopril , an angiotensin-converting enzyme inhibitor , or verapamil , a calcium channel blocker , would suppress the PDGF gene expression in stimulated human saphenous vein ECs .; inhibited by: enalapril [ pdgf01130.html ]

We have now assessed the effect of the angiotensin-converting enzyme inhibitor enalapril on the expression of the ET-1 , TNF-alpha , PDGF-B , TGF-beta , and bFGF genes in 24-week-old rat glomeruli after streptozotocin injection .
aromatase: inhibited by: something [ pdgf01401.html ]

These effects in both types of cultures were inhibited by platelet derived growth factor ( PDGF ) ( 20 ng/ml ) , a growth factor that up-regulates AR mRNA levels , and by fadrozole ( 100 nM ) , an aromatase inhibitor of the T/estrogen conversion .
cdk: inhibited by: something [ pdgf00491.html ]

Differential modulation of G1 cyclins and the Cdk inhibitor p27kip1 by platelet-derived growth factor and plasma factors in density-arrested fibroblasts .; inhibited by: something [ pdgf00491.html ]

Density-arrested fibroblasts contain low levels of cyclins E and A , and high levels of the Cdk inhibitor p27kip1 .
chemical: inhibited by: 2-aminopurine [ pdgf00509.html ]

Previous reports have shown that 1 ) dsRNA , a direct activator of PKR , induces the genes c-myc , c-fos , and JE ; 2 ) 2-aminopurine , a chemical inhibitor of PKR , blocks the induction of these genes by serum ; and 3 ) activated p21ras induces a cellular inhibitor of PKR .
cyclic nucleotide phosphodiesterase: inhibited by: something [ pdgf01497.html ]

The mitogenic effects of PMA were potentiated by cycloheximide pretreatment , and they were abolished by 3-isobutyl-1-methyl xanthine ( IBMX ; a cyclic nucleotide phosphodiesterase inhibitor ) .
cyclic phosphodiesterase: inhibited by: cilostazol [ pdgf01725.html ]

Effect of cilostazol , a cyclic AMP phosphodiesterase inhibitor , on the proliferation of rat aortic smooth muscle cells in culture .; inhibited by: Cilostazol [ pdgf01725.html ]

Cilostazol , a cyclic AMP phosphodiesterase inhibitor , has been used as an antiplatelet agent .
cyclin-dependent kinase: inhibited by: something [ pdgf00596.html ]

We have investigated the regulation of p27kip1 , a cyclin-dependent kinase inhibitor , in BALB/c 3T3 cells during growth factor-stimulated transition from quiescence ( G0 ) to a proliferative ( G1 ) state .
cyclooxygenase: inhibited by: indomethacin [ pdgf00304.html ]

The PDGF-stimulated increase in cAMP was prevented by addition of the cyclooxygenase inhibitor indomethacin , consistent with release of prostaglandins stimulating cAMP .; inhibited by: something [ pdgf00439.html ]

The cyclooxygenase inhibitors S , ibuprofen and indomethacin , were slightly inhibitory .
dna synthesis: inhibited by: something [ pdgf01471.html ]

To investigate whether expression of c-sis RNA is regulated in a cell cycle dependent manner , human A172 glioblastoma cells were synchronized by either centrifugal elutriation or chemical blockage with the DNA synthesis inhibitors S hydroxyurea or aphidicolin .
efficient serine/threonine protein kinase: inhibited by: K252a [ pdgf01764.html ]

K252a , an efficient serine/threonine protein kinase inhibitor ( IC50s of to 30 nM ) , has been shown to block the neuronal differentiation of rat pheochromocytoma PC12 cells induced by nerve growth factor ( NGF ) .
endomembrane ca2+ atpase: inhibited by: thapsigargin [ pdgf01140.html ]

However , the release of [ Ca2+]i from the intracellular store of the nef-expressing cells by an endomembrane Ca2+ ATPase inhibitor , thapsigargin , revealed that these cells contained normal Ca2+ stores .
growth: inhibited by: something [ pdgf00568.html ]

Transforming growth factor-beta ( TGF-beta ) acts as a growth inhibitor , yet it can stimulate proliferation ; 1-2 fg/cell of TGF-beta 1 elicits maximal proliferation of dense and sparse cultured smooth muscle cells ( SMCs ) , whereas higher amounts are less stimulatory .; inhibited by: something [ pdgf01715.html ]

Our results show that stimulation by serum of dense cultures of 3T3 cells rapidly induced increased synthesis of a growth inhibitor ( mIGFBP-3 ) capable of binding IGF .; inhibited by: something [ pdgf01923.html ]

With TGF beta , which is a growth inhibitor for certain epithelial and other cell types , the loss of the normal inhibitory response in transformed cells could have the same result as the activation of a growth-stimulatory response .; inhibited by: something [ pdgf01924.html ]

Thus TGF beta is primarily a growth inhibitor and not a classical growth factor .
growth factor: inhibited by: drug suramin [ pdgf01137.html ]

In contrast , we found that AII-stimulated mitogenesis could be inhibited in a dose-dependent manner by the growth factor inhibitor drug suramin .
hmg reductase: inhibited by: 25-hydroxycholesterol [ pdgf01932.html ]

It was found that short transient exposures to the HMG CoA reductase inhibitor 25-hydroxycholesterol temporarily blocked the cell cycle traverse in the postmitotic half of G1 ( G1pm ) , whereas cells in the subsequent cell cycle phases were unaffected .
hmg-coa reductase: inhibited by: lovastatin [ pdgf01575.html ]

Recipients treated with the HMG-CoA reductase inhibitor lovastatin , which inhibits isoprenoid production , showed significantly decreased allograft intimal area after weeks , when compared with untreated recipients .
kinase: inhibited by: staurosporine [ pdgf01512.html ]

The effects of the kinase inhibitor staurosporine on mitogenesis in NIH/3T3 fibroblasts were characterized .
lipoxygenase: inhibited by: something [ pdgf00401.html ]

The PDGF-induced signal transduction cascade was studied to determine the potential mechanism of action of the lipoxygenase inhibitors S .
lipoxygenase pathway: inhibited by: something [ pdgf01663.html ]

We have measured the effect of the lipoxygenase pathway inhibitors S nordihydroguiaretic acid ( NDGA ) , 5,6-dehydroarachidonic acid , and baicalein on bovine capillary endothelial cell ( EC ) and aortic smooth muscle cell ( SMC ) growth in the presence or the absence of growth factors .
natural tyrosine kinase: inhibited by: erbstatin [ pdgf01190.html ]

The natural tyrosine kinase inhibitor erbstatin and a synthetic analog ( 1302 ) were co0pared for their inhibitory activity on EGF receptor kinase , PDGF receptor kinase and a src-type kinase from bovine brain .
nonspecific lipoxygenase: inhibited by: something [ pdgf00401.html ]

Pretreatment with either the nonspecific lipoxygenase inhibitor ( nordihydroguaiaretic acid ) or specific inhibitors of 5-lipoxygenase ( SC-41661 and ICI-230487 ) inhibited PDGF-induced mitogenesis .
nucleoside transport: inhibited by: nitrobenzylthioinosine [ pdgf00548.html ]

Experiments with the nucleoside transport inhibitor nitrobenzylthioinosine suggested that the transport of c3Ado into EC was required for its inhibitory activity .
pdgf receptor-associated tyrosine kinase: inhibited by: something [ pdgf00142.html ]

Furthermore , the strain-induced stimulatory effect on DNA synthesis of fetal lung cells was blocked by tyrphostin 9 ( 1 microM ) , a PDGF receptor-associated tyrosine kinase inhibitor , but not by its inactive structural analogue tyrphostin 1 . Antisense but not sense PDGF-beta-R ON ( 10 microM ) also abrogated the strain-enhanced DNA synthesis .
phosphatase: inhibited by: sodium orthovanadate [ pdgf00226.html ]

The inhibitory effect of suramin on receptor conformation was abolished by the phosphatase inhibitor , sodium orthovanadate , suggesting that receptor phosphorylation mediated the conformational change .; inhibited by: sodium orthovanadate [ pdgf00277.html ]

The inhibitory effect of suramin on receptor conformation was abolished by the phosphatase inhibitor sodium orthovanadate , suggesting that receptor phosphorylation mediated the conformational change .; inhibited by: okadaic acid [ pdgf01648.html ]

We now report evidence that the serine/threonine kinase inhibitors H7 and H8 but not HA1004 , W-7 , and ML-7 inhibit the transcriptional induction of the JE gene by serum whereas the phosphatase inhibitor , okadaic acid , increases JE expression .
phosphatases: inhibited by: sodium orthovanadate [ pdgf01765.html ]

Phosphorylation of p175 is not induced by treatment of the cells with the phosphatases inhibitor sodium orthovanadate .
phosphodiesterase: inhibited by: IBMX [ pdgf00304.html ]

Incubating cells with PDGF and -isobutyl-1-methylxanthine ( IBMX , a phosphodiesterase inhibitor ) enhanced the accumulation of cAMP and PKA activity by an additional . 5-3-fold , whereas IBMX alone was essentially without effect .; inhibited by: something [ pdgf00457.html ]

Treatment with forskolin ( an activator of adenylate cyclase ) , IBMX ( a phosphodiesterase inhibitor ) , PGE1 , and isoproterenol stimulated accumulation of both cAMP and c-myc RNA , but no increase in either cAMP or c-myc RNA was seen with the inactive forskolin analog , 9-dideoxyforskolin .; inhibited by: -isobutyl-1-methylxanthine [ pdgf01464.html ]

Moreover , a phosphodiesterase inhibitor , -isobutyl-1-methylxanthine , and a more specific cAMP phosphodiesterase inhibitor , Ro 20-1724 , augmented the inhibition of DNA synthesis in SMC concomitant with further elevation of cAMP level .
phospholipase C: inhibited by: U-71322 [ pdgf00492.html ]

The chemotactic response of F5 met was inhibited by both the PI 3-kinase inhibitor wortmannin and the phospholipase C inhibitor U-71322 .
physiologic growth: inhibited by: something [ pdgf00680.html ]

None of these stimulated melanoma cell growth consistently , whereas exogenous , acid-activated TGF- beta inhibited melanoma growth at concentrations greater than 10 ng/ml , suggesting that bioactive TGF- beta may represent a physiologic growth inhibitor .
plasmin: inhibited by: Tranexamic acid [ pdgf00758.html ]

Tranexamic acid , a plasmin inhibitor , abrogated the stimulation of SMC migration by IL-4 .
potent growth: inhibited by: something [ pdgf00436.html ]

Transforming growth factor-beta 1 ( TGF-beta 1 ) is a potent growth inhibitor for many cell types .
potent phosphatase: inhibited by: Vanadate [ pdgf00160.html ]

Vanadate , a potent phosphatase inhibitor , induced a twofold increase in thymidine incorporation .; inhibited by: ocadaic acid [ pdgf01127.html ]

This effect could be augmented by the addition of ocadaic acid , a potent phosphatase inhibitor , suggesting that protein phosphorylation plays a role in this process .
potent phosphotyrosyl phosphatase: inhibited by: something [ pdgf01695.html ]

In the present study , we focus our investigation on the similarity between a redox cycling naphthoquinone and orthovanadate ( VO ) , an oxidant generator as well as a potent phosphotyrosyl phosphatase inhibitor , in growth of 3T3-L1 cells cultured in serum-free media .
potent protein-tyrosine kinase: inhibited by: something [ pdgf00507.html ]

We describe a potent protein-tyrosine kinase inhibitor ( CGP 53716 ) that shows selectivity for the PDGF receptor in vitro and in the cell .
powerful growth: inhibited by: something [ pdgf00879.html ]

Heparin is a powerful growth inhibitor for SMC .
prostaglandin h synthase: inhibited by: indomethacin [ pdgf00854.html ]

The addition of indomethacin , a prostaglandin H synthase inhibitor , or nordihydroguaiaretic acid , a lipoxygenase inhibitor , did not affect PDGF-stimulated thymidine uptake into DNA .
prostaglandin synthesis: inhibited by: indomethacin [ pdgf00359.html ]

IL-1 beta stimulates PDGF-AA synthesis also in the presence of indomethacin , a prostaglandin synthesis inhibitor .
protein kinase: inhibited by: something [ pdgf00447.html ]

The protein kinase inhibitors S , staurosporine and K252a , block PDGF-induced ISRE binding and -5A synthetase gene expression .
protein kinase C: inhibited by: Ro-31-8220 [ pdgf00185.html ]

Treatment of the cells with the protein kinase C inhibitor , Ro-31-8220 , and depletion of cellular protein kinase C by pretreatment with 4 beta-phorbol 12-myristate 13-acetate resulted in loss of PLD activation by PDGF indicating a PKC-dependent mechanism .; inhibited by: calphostin C. [ pdgf00298.html ]

However , stimulation of c-fos was slightly more sensitive to genistein , while the B2 receptor mRNA was more sensitive to inhibition by the protein kinase C inhibitor , calphostin C. The increase in cell surface B2 receptors were functionally coupled to an increase in phosphoinositide-specific phospholipase C , and the effects of PDGF were selective as there was no increase in either angiotensin II- or arginine vasopressin-induced inositol phosphate formation or intracellular calcium release .; inhibited by: staurosporin [ pdgf00303.html ]

The protein kinase C inhibitor , staurosporin , completely suppressed an increase in PDGF-BB binding by high glucose , and high glucose significantly activated protein kinase C. These results indicated that PDGF-beta receptor expression was enhanced by high glucose through the activation of protein kinase C. Furthermore , we observed similar effects of high glucose on both PDGF-beta receptor expression and protein kinase C activation in rat mesangial cells and human capillary endothelial cells .; inhibited by: GF [ pdgf00311.html ]

This decrease in proliferation as well as the change in morphology of the TPA-treated cells can be blocked by the protein kinase C inhibitor GF 109203X .; inhibited by: something [ pdgf00439.html ]

The protein kinase C inhibitors S , H7 and staurosporine , strongly inhibited the shear-induced PDGF gene expression in HUVEC .; inhibited by: something [ pdgf00617.html ]

The effects of the protein kinase C inhibitors S on proliferation could not be ascribed to cytotoxicity .; inhibited by: something [ pdgf01479.html ]

To evaluate the role of protein kinase C-mediated pathways in the proliferation of malignant gliomas , this study examined the effect of a protein kinase C ( PKC)-activating phorbol ester ( 12-O-tetradecanoyl-13-phorbol acetate or TPA ) and a protein kinase C inhibitor ( polymyxin B ) on deoxyribonucleic acid ( DNA ) synthesis of malignant glioma cells in vitro .
protein synthesis: inhibited by: cycloheximide [ pdgf00002.html ]

The effects of PDGF on PDGF-A mRNA and polypeptide levels were prevented by the protein synthesis inhibitor cycloheximide at 3.6 microM .; inhibited by: cycloheximide [ pdgf00165.html ]

PDGF A-chain mRNA levels increase when quiescent HUVE cells are treated with the protein synthesis inhibitor cycloheximide ; therefore , the effect of cycloheximide on PDGF A-chain mRNA decay was also investigated .; inhibited by: cycloheximide [ pdgf00218.html ]

HBGF-1 induction of PDGF A-chain mRNA expression occurs in the presence of the protein synthesis inhibitor cycloheximide and thus does not require de novo protein synthesis .; inhibited by: cycloheximide [ pdgf00772.html ]

A rapid increase in activity ( maximal at min ) is followed by a lower persistent level of activity which is maximal at 4-6 h . The second peak of MAP kinase activity is markedly attenuated by the protein synthesis inhibitor cycloheximide and , consequently , is paralleled by a marked de-novo synthesis of p42 and p44 MAP kinases , as measured by immunoprecipitation of [ 35S]methionine-labeled mesangial cells and by a 700% increase in total MAP kinase protein , as detected by Western-blot analysis .; inhibited by: something [ pdgf01208.html ]

The protein synthesis inhibitors S cycloheximide and puromycin reversed the glucocorticoid-mediated inhibition and suggested that new protein synthesis was necessary .; inhibited by: cycloheximide [ pdgf01295.html ]

The protein synthesis inhibitor , cycloheximide ( 10 micrograms/ml ) , also increases the level of urokinase mRNA .
protein-synthesis: inhibited by: cycloheximide [ pdgf00097.html ]

The presence of a protein-synthesis inhibitor , cycloheximide , in the culture medium did not significantly influence the PDGF A-chain mRNA level in normal mesothelial and malignant mesothelioma cell lines .
protein-tyrosine kinase: inhibited by: something [ pdgf00507.html ]

Selective inhibition of the platelet-derived growth factor signal transduction pathway by a protein-tyrosine kinase inhibitor of the -phenylaminopyrimidine class .
proteinase: inhibited by: something [ pdgf00111.html ]

In this study , we demonstrate that the proteinase inhibitor and cytokine carrier alpha 2-macroglobulin ( alpha 2M ) increases rat VSMC PDGF alpha-receptor expression .; inhibited by: something [ pdgf00197.html ]

Therefore , under normal physiological conditions , native alpha 2M ( non-alpha 2M-R/LRP-recognized ) is the primary form of the proteinase inhibitor functioning as a carrier of PDGF-BB and TGF-beta 1 in the blood .; inhibited by: something [ pdgf01921.html ]

In addition to its effects on the secretion of PA , TGF beta enhanced the production of a proteinase inhibitor by these cells .
reductase: inhibited by: something [ pdgf00762.html ]

A reductase inhibitor , we evaluated the effects of lovastatin on DNA replication and the proliferation of rat mesangial and aortic smooth muscle cells , both of which were mesenchymal origin cells .
rna ii: inhibited by: alpha-Amanitin [ pdgf00315.html ]

alpha-Amanitin , an RNA polymerase II inhibitor , blocked transcription by more than 70% .; inhibited by: 6-dichloro-1-beta-D-ribofuranosyl benzimidazole [ pdgf01639.html ]

In order to decide if the u-PAR mRNA increase was due to message stabilization or a consequence of transcriptional activation we used the RNA polymerase II inhibitor , 6-dichloro-1-beta-D-ribofuranosyl benzimidazole ( DRB ) during the stimulation experiments .
rna polymerase: inhibited by: 6-dichlorobenzimidazole riboside [ pdgf00596.html ]

Full repression of p27kip1 synthesis required the continued presence of PDGF and failed to occur in the presence of the RNA polymerase inhibitor , 6-dichlorobenzimidazole riboside .
rna synthesis: inhibited by: alpha-amanitin [ pdgf01865.html ]

The induction of TH by muscle extract was ( 1 ) dose dependent ; ( 2 ) paralleled by a proportional increase in the steady-state levels of TH mRNA ; ( 3 ) greatly reduced by the RNA synthesis inhibitor alpha-amanitin or the protein synthesis inhibitor cycloheximide ; and ( 4 ) unassociated with change in the survival of neurons in culture .
rna transcription: inhibited by: 5,6-dichloro-1 beta-D-ribofuranosylbenzimidazole [ pdgf00189.html ]

The data indicate that the t1/2 of PDGF-alpha receptor mRNA is approximately 3.3 h after incubation with the RNA II polymerase transcription inhibitor 5,6-dichloro-1 beta-D-ribofuranosylbenzimidazole ( DRB ) .
selective kinase C: inhibited by: Ro 31-8220 [ pdgf01302.html ]

A selective protein kinase C inhibitor , Ro 31-8220 , abolished induction of the 95 kDa gelatinase .
serine protease: inhibited by: inter-alpha-inhibitor [ pdgf01563.html ]

OBJECTIVE : The hyaluronan-binding protein TSG-6 ( tumor necrosis factor-stimulated gene 6 ) forms a stable complex with the serine protease inhibitor , inter-alpha-inhibitor , potentiates the inhibition of plasmin activity , and has antiinflammatory effects in vivo .
serine/ threonine phosphatase: inhibited by: NIH3T3 cells [ pdgf01545.html ]

In NIH3T3 cells , the serine/ threonine phosphatase inhibitor okadaic acid and the tyrosine kinase inhibitor genistein increased Shb mRNA contents , the protein kinase C activating phorbol ester 12-O-tetradecanoyl -acetate ( TPA ) decreased the Shb mRNA content , whereas the tyrosine kinase inhibitor tyrphostin 25 and the mitogen platelet-derived growth factor ( PDGF-BB ) had no effect .
serine/threonine protein kinase: inhibited by: H7 [ pdgf00086.html ]

The serine/threonine protein kinase inhibitor , H7 , blocked PDGF A and B chain mRNA induction in response to TGF-beta .
specific cam: inhibited by: W-7 [ pdgf01519.html ]

Trifluoperazine and W-7 , both specific CaM inhibitors S , similarly inhibited DNA synthesis stimulated by EGF , PDGF or FBS in a dose-dependent manner , whereas W-5 , a less specific CaM inhibitor , was minimally effective .
specific cytoplasmic protein tyrosine kinase: inhibited by: something [ pdgf01702.html ]

Herbimycin A , a specific cytoplasmic protein tyrosine kinase inhibitor , selectively inhibited the growth of src or abl transformed cells in the serum-free medium resulting in about 10-fold or fivefold lower IC50 than those in the serum-containing medium .
specific pkc: inhibited by: CGP [ pdgf00980.html ]

The late phase of the time-dependent epidermal growth factor ( EGF)-induced biphasic activation of the p70s6k is selectively attenuated by the specific PKC inhibitor , CGP 41,251 , a staurosporine derivative .
specific tyrosine kinase: inhibited by: something [ pdgf01762.html ]

Herbimycin A , a specific tyrosine kinase inhibitor , both decreases thrombin-induced mitogenesis by greater than 90% and abolishes tyrosine phosphorylation of phospholipase C ( PLC)-gamma-1 .
specific tyrosine protein kinase: inhibited by: genistein [ pdgf00566.html ]

The inductions were abolished when genistein , a specific tyrosine protein kinase inhibitor , was added concomitantly with PDGF , a condition in which DNA synthesis is known to be inhibited .
synthase: inhibited by: something [ pdgf01162.html ]

The effects of hypoxia and the NO synthase inhibitor on ET-1 expression are additive .
synthetic matrix metalloproteinase: inhibited by: something [ pdgf01072.html ]

Regulation of vascular smooth muscle cell migration and proliferation in vitro and in injured rat arteries by a synthetic matrix metalloproteinase inhibitor .
synthetic mmp: inhibited by: something [ pdgf01072.html ]

To assess the role of MMPs in SMC proliferation and migration and intimal thickening , we measured the effect of the synthetic MMP inhibitor BB94 ( Batimastat ) on DNA synthesis and migration of SMCs in vitro as well as the formation of a neointima after balloon injury to the rat carotid artery .
thromboxane synthetase: inhibited by: Dazmegrel [ pdgf00739.html ]

In order to examine ( indirectly ) the role of PG in mediating changes in MC DNA synthesis , indomethacin ( 1.5 x 10(-5 ) mol/l ) or the thromboxane A2 synthetase inhibitor Dazmegrel ( 10(-5 ) mol/l ) was added to the medium and DNA synthesis assessed after 72 h using 3H-thymidine incorporation ( 3H-TdR ) .
tissue: inhibited by: something [ pdgf01743.html ]

Most interestingly , the effects of EGF and TGF-alpha can be completely reversed by the tissue inhibitor of metalloproteinases , TIMP .
transcription: inhibited by: actinomycin D [ pdgf01816.html ]

The transcription inhibitor , actinomycin D , and protein synthesis inhibitor , cycloheximide , inhibited the increase in GM-CSF and G-CSF production induced by IL-1 and TNF .
tyrosine kinase: inhibited by: Genistein [ pdgf00160.html ]

Genistein , a tyrosine kinase inhibitor , inhibited in a dose-related manner ( 2-20 microM ) the mitogenic effect induced by either PDGF or the combination of vanadate and PDGF .; inhibited by: tyrphostin [ pdgf00298.html ]

Comparison with the immediate early response gene c-fos demonstrated that the increase in B2 receptor mRNA was similarly inhibited by the tyrosine kinase inhibitor , tyrphostin , as well as staurosporine .; inhibited by: genistein [ pdgf00542.html ]

In the present study , we investigated the effects of genistein , a tyrosine kinase inhibitor , and NiCl2 , a Ca2+ influx blocker , on PDGF-induced Ca2+ transient and on expression of c-fos and c-myc mRNA .; inhibited by: genistein [ pdgf00832.html ]

The tyrosine kinase inhibitor genistein dose-dependently antagonized the inhibitory effect of PDGF-BB on IL-1 beta-stimulated PLA2 secretion , thus suggesting that tyrosine phosphorylation may be required for PDGF-BB inhibition of PLA2 gene expression in mesangial cells .; inhibited by: herbimycin-A [ pdgf00948.html ]

In this communication , we demonstrate that the tyrosine kinase inhibitor herbimycin-A ( HM-A ) inhibited the effects of PDGF on SMCs in proliferation , phenotypic modulation , and reorganization of F-actin .; inhibited by: genistein [ pdgf00948.html ]

Another tyrosine kinase inhibitor genistein also inhibited reorganization of F-actin .; inhibited by: something [ pdgf00948.html ]

Since both tyrosine kinase inhibitors S reduced PDGF-dependent tyrosine phosphorylation , tyrosine kinase activities may be involved not only in proliferation but also in reorganization of F-actin .; inhibited by: tyrphostin [ pdgf01084.html ]

Growth factor-mediated induction of ScR activity in rabbit and human SMC was blocked by the tyrosine kinase inhibitor tyrphostin 47 , whereas the induction of ScR activity in rabbit but not human SMC was blocked by the protein kinase C inhibitor MDL.29,152 .; inhibited by: Genistein [ pdgf01110.html ]

Genistein , a tyrosine kinase inhibitor , inhibited PLD activation but not DGK activation , the latter was inhibited selectively by R 59022 .; inhibited by: herbimycin [ pdgf01371.html ]

PDGF-induced beta-actin mRNA redistribution was inhibited by the tyrosine kinase inhibitor herbimycin , indicating that this process requires intact tyrosine kinase activity , similar to actin filament polymerization and chemotaxis .; inhibited by: genistein [ pdgf01491.html ]

We have examined the effects of the tyrosine kinase inhibitor genistein on hormone dependent cell proliferation and intracellular signalling in mouse 10T1/2 fibroblasts and rat liver T51B epithelial cells .
tyrosine phosphatase: inhibited by: pervanadate [ pdgf00924.html ]

Treatment with the tyrosine phosphatase inhibitor pervanadate further increased PLC gamma 2 tyrosine phosphorylation as well as [ 3H]IP2 and [ 3H]IP3 generation .
tyrosine protein kinase: inhibited by: something [ pdgf00566.html ]

Inhibition of PDGF-induced c-jun and c-fos expression by a tyrosine protein kinase inhibitor .
ubiquitous proteinase: inhibited by: something [ pdgf00051.html ]

Alpha 2-Macroglobulin ( alpha 2M ) is a multifunctional secreted glycoprotein that serves as a ubiquitous proteinase inhibitor and as a binding protein for platelet-derived growth factor ( PDGF ) BB and homologues of PDGF-BB secreted in culture by macrophages .
weaker ace: inhibited by: something [ pdgf01185.html ]

Although tuna AI was a weaker ACE inhibitor than captopril , the increasing effect of tuna AI on the migration and the IL-1 generation in BAECs was slightly greater than that of captopril .